Coding

Part:BBa_K3819007:Design

Designed by: Yuanyi Wang   Group: iGEM21_UESTC-China   (2021-09-25)


EG1(Trichoderma sp. SSL endoglucanase I)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

GenBank: EU935217 SOURCE: Trichoderma sp. SSL TITLE: Cloning and expression of the endoglucanase I from Trichoderma sp.in Pichia pastoris AUTHORS: Huang,J.Z., Du,Z.P., Liu,H.Y., Shu,Z.Y., Zhu,Y.R. and Wang,J.


References